图1来自:发育过程中神经元存活的细胞适合度选择模型a我们工作假设的方案。b, c 4-OHT诱导TRKC PSNs的时间命运图谱。4-OHT注射2小时后，TrkCCreER小鼠允许TRKC+细胞中CreER的临时激活21,22。在E17.5 DRG切片上对PV、RFP和RUNX3进行免疫染色(c)，图显示PV+/RUNX3+ PSNs在TOM+细胞中的分布(n = 4)。比例尺:20 μm。d在C5和C7处的psn定量。***P < 0.001，采用Sidak多重比较检验进行单因素方差分析(ANOVA) (n = 2-3)。图示PSNs细胞死亡窗口。e TRKC在E11.5 ISL1+(和RUNX3+，其染色没有显示更多的可见性)DRG神经元中的表达。 Scale bar: 50 μm. f TRKC levels in PSNs of e illustrated by color coding; dark blue indicates the lower and red the higher TRKC levels. From here, all observations are done at brachial levels (C5–8). g Distribution of TRKC levels in PSNs from e. h Distribution of TRKC levels in PSNs in E11.5 DRG neurons (from g). The data exhibit a Poisson-like distribution (one representative animal), with the mean used to define the two different categories of TRKC intensity (TRKCHigh and TRKCLow). i Projection of seven images of RUNX3+/TRKC+ PSNs from one brachial DRG; dots indicate TRKC-labeled neurons and color codes reveal TRKC intensity as shown in h. j Projection image of smFISH for pan Ntrk3 and Ntrk3 full length (FL) transcripts in E11.5 DRG, visualized at high magnification in (1) and (2) (images show full projection); right panel shows color coding of Ntrk3 FL levels in red; the brighter, the higher levels. k Distribution of the number of Ntrk3 FL molecules in E11.5 DRG neurons by smFISH, normalized to pan Ntrk3 (Ntrk3 FL represent 68% of all Ntrk3 transcripts). l TrkCCreER;R26tdTOM mice were injected at E9.75 with 4-OHT and analyzed at E11.5 (n = 3). m, n Frequency distribution (m) and pie chart (n) of TOM+/TRKC+ neurons from l according to their level of TRKC intensity. Source data are available as a Source Data file
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