图1.缺血性WT视网膜中TNC的早期诱导。（A-D）TNC的图像在3和7天中从对照和缺血性WT组染色视网膜横截面。免疫组织化学在IPL和OPL以及NFL（红色）中揭示了一种TNC信号。细胞核与pro-3（蓝色）复位。（e）TNC +染色面积在3天后显着增加，表明缺血性损伤后的TNC早期诱导。然而，在7天内可以证明显着减少的TNC染色。WT CO组设定为100％。（F-I）一致地，相对蛋白质定量在I / R后3天显示出较大的TNC同种型显着上调。（J-M）7天后观察到相当的TNC蛋白水平。（n）在缺血后两点不注时地注意到TNC mRNA表达的差异。 Data were analyzed via Student t-test and presented as mean ± standard error mean (SEM) in panels (E,G–I,K–M). For RT-qPCR, groups were compared using the pairwise fixed reallocation and randomization test in panel (N). These data are shown as median ± quartile ± minimum/maximum. *p < 0.05; **p < 0.01. n = 4–5/group. Scale bar = 20 μm. ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer, NFL: nerve fiber layer. Credit:神经科学的前沿（2021）。DOI：10.3389 / FNINS.2021.642176
用户评论