图1所示。组织转谷氨酰胺酶(TG2)促进腹膜肿瘤生长在一个同源的卵巢癌(OC)小鼠模型通过防止CD8 + T细胞渗透到腹水。(A, B)的腹膜腹水(±SEM, n = 23每组,累计四个独立的实验数据如图所示)(A),和数字的细胞腹水(±SEM,每组(n = 5,数据从一个代表性的实验的两个执行如图所示)C57BL / 6 (B) (TG2 + / +)和TG2淘汰赛(TG2 - / -)雌性小鼠腹腔内注射后6周ID8细胞。t检验p值显示。(C)腹膜转移的图片TG2 + / +和TG2 - / -腹部蛀牙。肿瘤移植用蓝色箭头表示。(D) kaplan meier生存分析TG2 + / + (n = 12)和TG2 - / - (n = 14)小鼠腹腔注射ID8细胞。图代表了数据从一个实验的两个执行。(情况)的免疫细胞流式细胞仪测量的百分比TG2 + / +和TG2 - / -小鼠移植肿瘤诱导ID8腹腔接种细胞。(E) CD8 +及CD4 + T细胞在脾脏(TG2 + / + n = 10; TG2-/-, n=9; data from two experiments). (F) CD8+ and CD4+ T cells in abdominal ascites (TG2+/+, n=13; TG2-/-, n=16; data from four experiments). (E, F) Programmed cell death protein 1 (PD-1) expressing CD8+ T cells in spleen (n=7 per group; data from one representative experiment) and ascites (n=9 per group; data pooled from three experiments). Values are means ± SEM (*p<0.05; **p<0.01). (G, H) T regulatory cells (Tregs) in spleen (n=10 per group; data from two experiments) and ascites (n=13 per group; data from three experiments). Activation marker expressions from one representative experiment are shown (n=4–7 per group). (I) Absolute cell counts in ascites (TG2+/+, n=6; TG2-/-, n=5). Total cell counts were used for determination of total cell number for each subset from data retrieved from fluorescence activated cell sorting (FACS, ****p<0.0001). Credit: DOI: 10.1136/jitc-2021-002682
