图1所示。减少5 -对自噬的影响在整个结肠DSS-induced结肠炎的老鼠。DSS(5%)给Tph1 + / +和Tph1−−/老鼠饮用水中5天诱导结肠炎。对照组收到热压处理过的饮用水。每组包含三到五只老鼠。(一)戴。* P < 0.05之间colitic Tph1 + / +和Tph1−−/老鼠天3到5。(B)宏观损伤评分后第五天DSS和控制老鼠。(C)免疫印迹图像和(D)自噬蛋白LC3的量化,Beclin-1 Atg12-5, p62。(E)免疫印迹图像和(F)的量化p-AMPKα(T172)和p-mTOR (S2448)。 Representative Western blot with β-actin is presented as loading controls. The blot shown is representative of three to five random mice in each group. (G) Representative micrograph. Arrows indicate LC3B, LAMP2, and double-stained IECs. Scale bars, 100 μm. (H) Percentage of LC3B+ and (I) percentage of LC3B/LAMP2+ double-stained IECs in colonic sections immunostained for LC3B and LAMP2. Bar graph represents means ± SEM. *P < 0.05 for graphs (B), (D), (F), (H), and (I). Credit: DOI: 10.1126/sciadv.abi6442