图1所示。影响的ephoss csc-associated细胞表面标记和stemness-associated基因表达水平。(一)实验设计的示意图。阶段1 (PT、原发肿瘤)涉及处理physioxia下的肿瘤和周围空气,流式细胞术特征的细胞,细胞传播,再植术的女性FVB / N小鼠细胞。第二阶段(PL、初级线)涉及流式细胞仪鉴定培养细胞的第一阶段。第三阶段(TT,移植肿瘤)涉及recharacterization肿瘤细胞植入的第一阶段获得的。第四阶段(TL,移植行)涉及流式细胞术特征从第三阶段肿瘤细胞生长。PT,原发肿瘤;PL,一次线;TT,移植瘤; TL, Transplanted Line. (B) Representative flow cytometry profile of tumor cells stained for antibodies against LGR5 and TSPAN8. Antibodies against lineage markers CD31-PE (phycoerythrin)/Cy7, CD45-PE/Cy7, and CD140a-PE/Cy7 were used to label endothelial cells, hematopoietic cells, and fibroblasts, respectively, and only lineage-negative cells were included in the analysis. (C) Quantitation of LGR5+ cells. Differences in LGR5+ cells between ambient air and physioxia are significant [n = 3 to 6, one-way analysis of variance (ANOVA)]. (D) CD61/TSPAN8 staining patterns of tumor cells (n = 3 to 6, one-way ANOVA). (E) Quantitation of TSPAN8+ cells. (F) Quantitation of CD61+ cells. (G) Tumor cells collected and processed at physioxia express higher levels of stemness-associated genes compared to ambient air (n = 3, one-way ANOVA, P = 0.0004). *P < 0.05, **P < 0.01, and ***P < 0.001 by ANOVA. ns, not significant. Credit: DOI: 10.1126/sciadv.abh3375