图1:不整合蛋白质组和转录组签名揭示潜在的平移控制机制在SC quiescence-to-activation过渡。工作流的示意图说明FACS-isolated SCs的蛋白质组学分析。SCs是排序从Pax7-nGFP老鼠。qsc排序从0.5% PFA-perfused老鼠。古罗马国库从unperfused老鼠被排序。对asc受伤(关于)排序从3天1.2%氯化钡受伤的老鼠。培养对asc (casc) 2天培养的。(n = 2独立实验)。b蛋白表达的层次聚类(光谱)数量的qsc,古罗马国库,常设委员会,公司。c的热图代表表达的细胞周期,静止,qsc activation-specific蛋白质,古罗马国库,常设委员会,公司。 d Heatmap of representative expression for fatty acid oxidation and glycolysis-related proteins of QSCs, fiSCs, iASCs, and cASCs. e Heatmap of representative expression for transmembrane protein/receptors of QSCs, fiSCs, iASCs, and cASCs. f, g Analysis of transcriptome and proteome on QSCs and cASCs (proteome comparison) and iASCs (transcriptome comparison). f Scatter plot of the fold change of RNA and protein comparing QSCs to ASCs. Heatmap shows the RNA and protein expression of representative genes in the lower right quadrant of the scatter plot. g Functional enrichment analysis of genes from the lower right quadrant of f by g:Profiler. The g:Profiler uses a hypergeometric test to measure the significance of functional terms in the input gene list. Credit: DOI: 10.1038/s41467-022-28612-1
