hiv - 1二聚作用的分析。一、二聚是hiv - 1生命周期中的一个关键步骤。单体的RNA被认为是优先翻译,与二聚的RNA,包装成病毒粒子的先决条件。通过复合二聚的RNA有助于维持基因组的完整性。b, hiv - 1 5′UTR由不同的结构域与hiv - 1生命周期不同的功能。焦油代表转录。聚(代表5′UTR聚腺苷酸化是不活跃的。U5独特的5个地区或PBS,代表主机tRNA的退火发起反转录。SL1-SL3包含包装的信号。SL2包含剪接提供。 Dimerization occurs through a kissing loop interaction at a sequence in SL1. LDIs/alternative folds involving LDIs, such as between SL1–U5 and U5–AUG may regulate dimerization. c, FARS-seq. Mutant RNA sequences are generated by mutagenic PCR and in vitro transcription. Mutant populations are physically separated into monomer and dimer fractions and probed with DMS or left untreated. Mutation frequencies are analyzed by next generation sequencing. d, Functional profiles are obtained by mutational interference. Kdimer is a quantitative measure of dimerization based on the ratio of mutations in the dimer selected versus monomer selected population, corrected for mutations introduced during the library preparation and sequencing. e, Structural profiles are obtained by DMS that specifically reacts with unpaired A and C residues. DMS-MaPseq measures DMS reactivities as mutation rates in DMS treated versus untreated controls. f, Two-dimensional analysis identifies RNA stems through correlations between stem-disrupting mutations and mutations induced by DMS. Credit:《自然结构和分子生物》上(2022)。DOI: 10.1038 / s41594 - 022 - 00746 - 2
