检查参与组成分泌腺BCSC压缩干细胞池大小。流式细胞仪分析ALDH +或ALDH−细胞的比例增长后FACS-sorted ALDH +或ALDH−T47D细胞。b的比例ALDH的流式细胞仪分析+ BCSCs RFP-labeled T47D细胞培养无标号ALDH−, ALDH +或父母T47D细胞。c的比例ALDH的流式细胞仪分析+ BCSCs T47D细胞培养与CM源自ALDH +, ALDH−,或父母的细胞。d条件培养液的示意图和transwell培养系统。e, f MCF-7或T47D细胞被mammosphere-enriched培养BCSCs或亲代细胞48 h,并具备干细胞属性被ALDEFLUOR随后分析化验(e)或mammosphere-formation化验(f)。酒吧规模:500μm。g h MCF-7或T47D细胞培养与各自的CM源自mammosphere-enriched BCSCs或亲代细胞48 h,并具备干细胞属性被ALDEFLUOR随后分析化验(g)或mammosphere-formation化验(h)。酒吧规模:500μm。我co-implantation模型的示意图。j, k的一系列限制稀释MCF-7-luc细胞与无标号4×105 mammosphere-enriched co-implanted BCSCs或亲代细胞进入主机老鼠。生物荧光成像(BLI)进行MCF-7-luc生成的肿瘤细胞(j), CSC频率计算使用ELDA软件(k)。结果显示为±s.d *P less than 0.05; **P less than 0.01; ***P less than 0.001; ns not significant (One-way ANOVA followed by Tukey’s multiple comparison test in (b, c) others unpaired two-tailed Student’s t test). Credit:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 29018 - 9
