CC内支架充当维持MTD凝聚力的结构拉链。(a)扩展的光感受器说明了相对于POC5信号的3个位置的微管蛋白宽度的测量。0:CC内支架的远端;+150:距CC内支架端的150 nm;-150:150 nm近端到CC内支架端。比例尺:200 nm。(b)从p4到p60的(a)(-150 nm,0 nm,+150 nm)在(a)(-150 nm,0 nm,+150 nm)中的3个位置的光感受器的微管蛋白宽度测量值;每个时间点≥3只动物。(c)描述了(d)中使用的母亲中心近端端与CC内部支架信号端(绿色)或MTDS扩散(d)中的CC内部支架信号端(绿色)之间的距离的测量方案。(d)比较CC内支架端(POC5)或微管扩散的位置相对于Centriole的近端端(从P4到P60)的比较; ≥3 animals per time point. Note that inner scaffold measurements correspond to the POC5 data presented in Fig 2F. (e) EM transversal sections at the CC (top) or at the bulge region (bottom). Filled green arrowhead points to the CC inner scaffold; empty green arrowhead reveals the absence of the CC inner scaffold. Scale bar: 200 nm. (f, g) Distribution of the perimeter (f) or circularity (g) of the MTDs from transversal sections of photoreceptor CC (gray) or bulge (orange). N = 1 animal. Means and standard deviations are listed in S1 Table. The data underlying all the graphs shown in the figure are included in the S1 Data file. CC, connecting cilium; EM, electron microscopy; MTD, microtubule doublet. Credit:PLOS生物学(2022)。doi:10.1371/journal.pbio.3001649