fbxl-1 (ok3741)蠕虫受损神经运动的功能。(A)咽泵率实验概述(补充视频1)。WT秀丽隐杆线虫动物解剖显微镜下观察使用斜照明;grinder看起来象一个白点(箭头)终端灯泡。(B)咽抽水率fbxl-1 (ok3741)和WT蠕虫在1 -日-成年期(n = 20 /应变在天成人阶段;n = 25 /应变在日成人阶段)。Bonferroni的校正方法应用于占多重比较,仍持有和重大的发现。(C) fbxl-1 (ok3741)和WT身体弯曲率在琼脂板(补充视频2和3)。n = 25蠕虫/应变;5生物复制。(D-F)蠕虫游泳活动分析液体媒体。(D和E)五个蠕虫被转移到每一滴液体媒体(比例尺:1毫米)载玻片。 Activity of all 5 worms in aggregate was recorded, and pixel change was analyzed using ZebraLab. (F) fbxl-1(ok3741) worm swimming activity was significantly lower than WT worms (Supplemental Videos 4 and 5). Each dot indicates the total activity of 5 worms in a single technical replicate. n = 12 for WT; n = 11 for fbxl-1(ok3741); with a total of 60 WT and 55 fbxl-1(ok3741) worms analyzed across 4 biological replicates. (G–J) Thrashing activity assay. (G) Each fbxl-1(ok3741) and WT worm was transferred to 5 μL liquid media for individual worm activity analysis by wrMTrck plugin (ImageJ). The graph shows the BBPS, which were significantly decreased in fbxl-1(ok3741) worms as compared with WT worms. Each dot indicates activity of an individual worm. n = 36 each strain. (H and I) Representative traces of worm movements detected by wrMtrck analysis. fbxl-1(ok3741) worms showed less activity and uncoordinated movements than WT worms. (J) Higher-magnification images of representative individual fbxl-1(ok3741) and WT worm traces. Data are shown as the mean ± SD. Significance was determined using unpaired Student's t test. *P < 0.05, **P < 0.01, ***P < 0.001. Credit:江森自控的洞察力(2022)。DOI: 10.1172 / jci.insight.156346
