福尔马林暴露的突变特征。仅C> T FFPE突变计数随福尔马林固定时间增加。我们观察到研究1（固定组）的未修复和修复的FFPE样品的增加。仅FFPE的突变是指仅在FFPE中发现的突变，但在FF样品或已知的种系数据库中没有发现。条高度代表n = 3例患者的平均C> T计数，并且单个计数被标记为黑点。b使用80通道频谱（非T> C）观察到无需修复的FFPE和修复的FFPE样品的一致和可分离的突变模式。我们使用T-SNE聚集了研究1和2的归一化80通道突变曲线（n = 110）（请参阅方法）。C没有观察到T> C突变的一致和可分离的突变模式。我们使用T-SNE聚集了研究1和2的归一化T> C突变曲线（n = 110）。d我们派生的FFPE特征与已知宇宙SBS特征的比较。 e, f Unrepaired signature is highly similar to SBS30 (e) and repaired signature is highly similar to SBS1 (f). We treated T>C features as missing data due to the strong batch-effect found in study 1, which is also observed in a few other studies shown in Supplementary Table 1 and therefore they were assigned to zeros. We noted that zero values are approximately close to the true T>C mutation probabilities in FFPE datasets without this batch-effect (Supplementary Fig. 6f). Error bars indicate the standard deviation in n = 55 independent samples with top 50% density in t-SNE cluster (see Methods). g, h Large variability in T>C mutation channels. We derived the T>C patterns using the same methods applied in (e, f). The error bar showed the standard deviations in n = 55 independent samples with top 50% density within the t-SNE (see Methods). Credit:自然通讯（2022）。doi：10.1038/s41467-022-32041-5
