成年期急性IRP消融会导致红细胞减少和骨髓减少。P1/2-CTR和P1/2-KO在第1天和第3天接受他莫西芬诱导IRP消融。第10天进行分析。(A) P1/2-KO小鼠组织中Irp1 (Aco1)和Irp2 (Ireb2)等位基因的基因组聚合酶链反应(PCR)分析。标记了floxed (flox)和truncated (Δ)等位基因。(B) RBC参数(P1/2-CTR, n = 16;P1/2-KO, n = 18)。血红蛋白,血红蛋白;MCV:平均红细胞体积;MCHC,平均红细胞血红蛋白浓度。 (C) Hepatic and splenic iron levels (n = 11). (D) Serum iron parameters (n = 10). (E) RBC decay markers (n = 10). (F) Serum levels of EPO (P1/2-CTR, n = 18; P1/2-KO, n = 17) and hepcidin (P1/2-CTR, n = 18; P1/2-KO, n = 16). (G) Spleen index = √[(100 × spleen weight in milligrams per body weight in grams)] (n = 7), and reticulocyte frequency in peripheral blood (PB) (P1/2-CTR, n = 7; P1/2-KO, n = 9). A.U., arbitrary units. (H) White blood cell (WBC) and platelet counts in PB (P1/2-CTR, n = 16; P1/2-KO, n = 18). (I) Flow cytometry (FCM) analysis of major WBC populations in PB. The gating strategy is shown on contour plots on the left. The histograms display cell counts for 3 × 105 events recorded (n = 8). (B to I) The results are presented as box plots (minimum to maximum values). Unpaired, two-tailed t test between P1/2-CTR and P1/2-KO. *P < 0.05; **P < 0.01; ***P < 0.001. APC, allophycocyanin; PE, phycoerythrin. Credit:科学的进步(2022)。DOI: 10.1126 / sciadv.abq4469