在清醒行为小鼠的DLS中，光标记的单质神经网络的全细胞记录。实验装置的示意图用optopatcher在小鼠DLS中进行全细胞记录，用于在线光遗传分类，同时在S1中进行LFP记录。通过独立传递到同侧和对侧胡须的空气泡刺激胡须，并使用非接触式红外led光电二极管监测胡须行为。b使用D1- chr2 - yfp(标记dMSNs，左)或D2- chr2 - yfp(标记iMSNs，右)小鼠(D1 n = 15只小鼠，D2 n = 19只小鼠)获得msn的光标记。D1-ChR2-YFP小鼠的dMSNs到SNr的典型投影以及D2-ChR2-YFP小鼠的iMSNs到GPe的典型投影是明显的。比例尺，1毫米。c显示Bregma全细胞和LFP记录位置的示意图(左)。图片和放大的插图(右)显示了一个例子(来自34个独立的神经元，结果相似)，在行为正常的小鼠体内全细胞记录下，DLS中充满生物素的MSN。插图显示了同样的细胞在更高的放大倍数下。 d Opto-tagging of MSNs using the optopatcher. Depolarizing responses to photostimulation of a positive cell (ChR2 + ) in D1-ChR2-YFP mouse (top) for the duration of the stimulation. Negative cells (ChR2 − , bottom) did not respond with depolarization to photostimulation. e Example of spontaneous membrane potential activity in an opto-tagged iMSN, whole-cell recorded in the DLS of an awake behaving mouse. Membrane potential (Vm, red) of the neuron was recorded simultaneously with measurement of whisker activity (Whisker, green), air puff stimulation indicated in gray (ipsi/contra). f Synaptic responses of the neuron showed in e, to contra- and ipsilateral whisker deflection. g Depolarization of the membrane potential preceded whisker movement in the same neuron showed in e. h Venn diagram showing the number of MSNs responding to whisker stimulation (turquoise circle) and to spontaneous whisking movement (pink circle) or absence of both (red circle). LFP Local Field Potential, Barrel field somatosensory cortex S1, IR infrared light, SNr Substantia Nigra pars reticulata, GPe Globus Pallidus externa, Ctx Cortex, WM White Matter, St Striatum, ChR2 Channelrhodopsin, a.u. arbitrary units. Credit:自然通讯(2023)。DOI: 10.1038 / s41467 - 023 - 36648 - 0
卡罗林斯卡学院的研究人员一直在研究运动是如何塑造我们的感官的，以及感觉和运动过程是如何在帕金森氏症中受到影响的。一项研究发表在自然通讯， de la Torre-Martinez等人的研究表明，运动减少了对感觉输入的反应，并且在帕金森病的动物模型中，感觉和运动过程都发生了改变。
