(A) Clinical score of Cd4creAhrfl/fl and Ahrfl/fl mice cohoused (females; representative plot includes n = 9 mice/group; total replicates of N = 2 experiments). Spinal cords sections of Cd4creAhrfl/fl and Ahrfl/fl were stained with Luxol fast blue and hematoxylin/eosin stain at day 31 post EAE induction (B) Representative images and (C) quantification of myelin stain. Images from 4 equally spaced spinal cord levels were averaged for each mouse. (n = 5 mice/group; unpaired t test p = 0.0706) (D) Clinical score of Cd4creAhrfl/fl and Ahrfl/fl littermate controls separated at weaning (3 weeks of age). (Females; representative plot includes n = 8–9 mice/group; total replicates of N = 2 experiments; Mann–Whitney U test on total scores reported in legend [p = 0.0096] and on single days reported on plot) (E) Luxol fast blue with hematoxylin/eosin stain at the peak stage of EAE (day 16) and at chronic phase (day 31). (F) Quantification of myelin stain by Luxol fast blue alone in Ahrfl/fl mice and (G) Cd4creAhrfl/fl mice. (n = 7–8 mice/group; N = 2 experiments; unpaired t tests [p = 0.8343, 0.0322]) Scale bars represent 400 μm. Error bars represent standard error from the mean. Credit: PLOS Biology (2023). DOI: 10.1371/journal.pbio.3002000">
单独安置的Cd4creAhrfl / fl从EAE中恢复，慢性期髓鞘染色增加。(A) Cd4临床评分creAhrfl / fl气道高反应性和fl / fl同室小鼠(雌性;代表性图包括n = 9只小鼠/组;N = 2次试验)。Cd4细胞的脊髓切片creAhrfl / fl气道高反应性和fl / fl在EAE诱导后第31天用Luxol耐蚀蓝和苏木精/伊红染色(B)代表性图像和(C)髓鞘染色定量。每只小鼠平均4个等间距脊髓水平的图像。(n = 5只/组;(D) Cd4的临床评分creAhrfl / fl气道高反应性和fl / fl窝仔对照在断奶(3周龄)时分开。(雌性;代表性图包括n = 8-9只小鼠/组;重复次数N = 2次;(E) Luxol耐蚀蓝与苏木精/伊红染色在EAE高峰期(第16天)和慢性期(第31天)进行Mann-Whitney U检验(图例中报告的总评分[p = 0.0096]和图例中报告的单天评分)。(F)在Ahr中单用Luxol耐蚀蓝定量髓鞘染色fl / fl小鼠和(G) Cd4creAhrfl / fl老鼠。(n = 7-8只/组;N = 2次实验;未配对t检验[p = 0.8343, 0.0322])比例尺为400 μm。误差条表示来自均值的标准误差。信贷:公共科学图书馆生物学(2023)。DOI: 10.1371 / journal.pbio.3002000
