SeV感染诱导APOBEC3B定位于应激颗粒。用SeV感染经DOX处理的U2OS-A3B-flag细胞(MOI = 1,24 hpi)，用flag和G3BP1抗体分别监测A3B和G3BP1的定位。比例尺:5 μm。b在指示的MOI下，在SeV感染的单个细胞中定量细胞的细胞质A3B病灶数量，持续24小时。最高;A3B灶的细胞百分比。红线表示平均值。(细胞数，n = 500) c经DOX处理和SeV感染的U2OS-A3B-flag细胞中A3B (Flag)和EDC4的免疫荧光(MOI = 1,24 hpi)。比例尺:5 μm。d U2OS细胞转染GFP标记的A3B构建物，随后感染SeV (MOI = 1)。 A3B localization to SGs was monitored 24hpi by immunofluorescence. Scale bar: 5 μm. e The localization of A3A-GFP to SGs after SeV infection at MOI = 1 was monitored by immunofluorescence at 24hpi with GFP and G3BP1 antibodies respectively. Scale bar: 5 μm. Immunofluorescence for A3B (Flag) and G3BP1 in U2OS-A3B-flag cells treated with DOX and transfected with poly(I:C) (200 ng/mL, 16 h) (f) or treated with NaAsO2 (250 µM, 1 h) (g). Scale bar: 5 μm. h U2OS cells were infected with SeV (MOI = 1), and indicated proteins and phosphorylation levels were monitored 24hpi by western blotting. i Quantification of the number of cytoplasmic A3B foci in individual U2OS cells knockdown for MAVS or PKR for 48 h and infected with SeV (MOI = 1, 24 hpi). Top; percentage of cells with A3B foci. Red lines indicate the mean. (Number of cells, n = 500). Quantification of the number of G3BP1 foci by cell in PKR knockdown cells (j) or cytoplasmic A3B foci by cell (k) in U2OS-A3B-Flag WT or PKR KO cells infected with SeV (MOI = 1, 24hpi). Top; Total percentage of cells with G3BP1 or A3B foci. Red lines indicate the mean. (Number of cells, n = 500). Credit:自然通讯(2023)。DOI: 10.1038 / s41467 - 023 - 36445 - 9
