触摸屏自行塑成任务评估巴甫洛夫的方法对reward-predicting刺激行为。自行塑成触摸屏操作室的布局描述两个屏幕(左,CS -;c +)和奖励杂志(RM)交付草莓奶昔奖励(10μl)。每个房间都配备了红外线photobeam(出生)启动试验,和前面两个红外photobeams(杉木)两侧的RM CS屏幕记录方法。红外线photobeam RM(不显示)记录延迟时间内收集的回报。b的流程图概述自行塑成任务期间收购(左)和逆转(右)训练。(左)在一个变量来审判后开始打破BIR紧随其后的表示刺激(c +或CS−)在10年代。在c +抵消一次奖励是交付,并开始一个新的来发现鼠标拉着离开了RM。在CS−抵消,没有奖励了,和一个新的来开始。在一个会话中,c +和CS−试验交替伪随机。 In total, each session ended after 20 CS+ and 20 CS- trials or after 60 min, whichever occurred first. (right) Following 10 acquisition sessions (1 session/day), mice undergo a total of 10 reversal sessions, in which the location of the CS+ and CS- were reversed. c (left) In contrast to the previous, both CS screens (left and right) had 50% of probability to deliver rewards in non-deterministic trials. Contingencies after CS+ or CS- remained similar as previously described. Within a single session a total of 20 CS+ and CS- trials were presented. (right) After 10 consecutive non-deterministic training sessions, mice followed 10 consecutive deterministic training sessions as described in (b). Figure 1a was created with BioRender.com. Credit:自然通讯(2022)。DOI: 10.1038 / s41467 - 022 - 35601 - x