成像质谱分析小鼠临界大小的股骨骨折治疗体外改造人类软骨基质细胞衍生光盘。(D)分割地图生成的质荷(m / z)值光谱BMSC-derived的整个组织的部分(A和B)和ATSC-derived (C和D)移植组使用一个无监督平分k - means算法。部分用不同的颜色代表不同的蛋白质组学在整个集群组织部分。前两个蛋白质组群区分骨(黄色)和肌肉组织(蓝色)。骨地区分割聚类识别两个额外的BMSC组(B)段和三段电视集团(C)。第三电视集团的蛋白质组集群(黄色)对应于断裂地区和中没有检测到BMSC组。额外的断裂区域分割显示两个不同的集群BMSC组(B插入放大表示),但只有一个集群在电视组(C插入放大显示)。(E)使用自底向上的液体chromatography-tandem质谱方法,几个m / z值被分配给COL1A1-specific肽。肽质谱显示表示m / z值的绝对强度的总骨骨折(黑线)和地区(灰色线)。蓝色阴影表示COL1A1肽(m / z = 2055)之间高度保守的人类和老鼠,而黄色阴影表明人类独一无二COL1A1肽(m / z = 2057)。(F) Movat的5 c染色完成包含BMSC-derived软骨瓣移植小鼠股骨骨折,放大(L)。(G)的高度保守的COL1A1肽(m / z = 2055)(总=人类+鼠标; colored green) was distributed throughout the bone of the BMSC group. (H) The human-specific COL1A1 peptide (m/z = 2057; colored red) shows a high intensity distribution exclusively in the fracture region. (I) Merge of the total and the human-specific COL1A1 peptide. (J) Merging the intensity distribution of the human-specific COL1A1 peptide (m/z = 2057) with Movat’s 5C staining shows that it is colocalized only with the area of remaining hard callus. (K) Magnification of Movat’s 5C staining of the same fracture region shown in (F), and (L) the corresponding merge with intensity distribution of the human-specific COL1A1 peptide (m/z = 2057). n = 4 biological replicates per group. Credit:科学转化医学(2023)。DOI: 10.1126 / scitranslmed.abm7477