实验计划和挑战后的体重减轻。(A)实验时间表。用LVS ΔcapB载体(ΔcapB)或rLVS ΔcapB/MN (ΔcapB/MN)疫苗免疫叙利亚仓鼠(8只/组,母4只,公4只),在第0、3、6周分别免疫ID、IN、SQ、PO 3次;第10周用104 PFU的SARS-CoV-2 (2019-nCoV/USA-WA1/2020株)向IN发起挑战;每天密切监测感染的临床症状,包括体重减轻。一半的动物在挑战后3天(dpi)对肺部病毒滴度实施安乐死;另一半在7 dpi时安乐死进行肺组织病理学检查。(B)挑战后体重变化。0 ~ 3 d, n = 8/组;第4 ~ 7天,n = 4/组。 Data are mean percent weight loss ± standard deviation. Mean % changes were compared among groups on each day using a repeated measure (mixed) analysis of variance model since observations on the same animal over days are correlated. P values for comparing mean changes were determined to be significant using Tukey’s adjusted criterion: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001 (black asterisks, versus unvaccinated; red asterisks, versus vector control). Normal quantile plot examination of the residual errors and the Shapiro-Wilk test (W = 0.984) confirmed that the data followed a normal distribution, allowing the use of a parametric model. Credit:微生物学领域(2023)。DOI: 10.1128 / spectrum.05035-22