RNA编辑分析BrainGVEX队列的概述。(A)的总结编辑网站中发现的每一步RNA编辑分析。从上到下:所有检测到的rna dna差异(抽样),常见的非零的网站编辑在≥10%的样本,SCZ之间不同的网站编辑和控制(见材料与方法),和重新编码网站的微分网站产生的蛋白质。样本总数N = 132,包括在分析中。(B)的每种类型的抽样比例一般网站中每个样本检测。(C)迪亚/样品分离条件所有微分网站覆盖了至少五总读。通过Wilcoxon等级P值的计算和测试。(D)微分编辑网站的层次聚类(行)和样品(列)。每个网站在所有样本z分数计算。(E) SCZ平均微分编辑网站的编辑水平和控制。 Numbers (N) of editing sites that were up- or down-regulated in SCZ are shown, which were compared via chi-square test (P value shown at the top). (F) Editing levels in controls or SCZ of each differential protein recoding site. Error bars correspond to the SEM. (G) Proportion of genes with ≥5 TPM in each cell type among all differentially edited genes in the single-nucleus RNA-seq data. Ex, excitatory neurons; In, inhibitory neurons; Olig, oligodendrocytes; Ast, astrocytes; OPC, oligodendrocyte progenitor cells; Mic, microglia; End, endothelial cells. (H) Proportion of observed editing sites in each cell type among all differentially edited sites. Cell type abbreviations are the same as in (G). Credit:科学的进步(2023)。DOI: 10.1126 / sciadv.ade9997
